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  • Introduction
  • Single Molecule, Real-Time (SMRT) DNA Sequencing
  • Key Feature: Long (and Very Long) Reads
  • Highly Accurate Sequencing Results
  • 2nd Gen Sequencing
  • SMRT® Sequencing - Errors are Random
  • SMRT® Sequencing Accuracy
  • Validation of Illumina SNPs with SMRT® Sequencing
  • The Importance of Mapping for Sequencing Accuracy
  • Example: Genomic Regions Associated with Mental Retardation
  • Comparison PacBio® Data to PCR-Sanger Validation
  • The Importance of Mapping for Sequencing Accuracy
  • Long Readlenghts for De Novo Human BAC Assemblies
  • PacBio® Reads Indicate Errors in the Sanger References
  • PacBio® Sequences Through DNA Palindromes
  • PacBio® Assembly Resolves the Palindrome Junction in Difficult to Assemble Plant BAC
  • Even Short Palindromes May Cause Misassemblies
  • Traditional Workflow for HLA Typing
  • Full Phasing Information for HLA Haplotyping
  • Long-Read SNP Phasing
  • Announcing...Reads with Extraordinary Lengths (XL)
  • XL Sequencing Performance
  • CSHL collaboration: Sequencing Rice with XL/C2
  • XL Sequencing Performance
  • Customer Example: PacBio® Long Reads to Scaffold Contigs
  • DNA Base Modifications are Important in:
  • Kinetics in Single-Molecule, Real-Time DNA Sequencing
  • Detecting Base Modifications with SMRT® Sequencing
  • Technology Roadmap
  • Improvements through Magnetic Bead Loading
  • Magnetic Bead Loading
  • Photodamage Mitigation: Photo-Protected Analogs
  • PacBio® RS Throughput Increase
  • Upcoming Improvements
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  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio