Click to advance to topic of interest

Up Arrow
  • Introduction
  • Outline
  • Transcript Diversity
  • Drosophila DSCAM Gene – 38,000 Isoforms
  • One Gene, Two Isoforms with Opposite Effects
  • Current State of Transcript Assembly
  • Determination of Transcript Isoforms
  • The Iso-Seq Method for High-quality, Full-length Transcripts
  • Detailed Clontech Workflow for Conversion of cDNA into SMRTbell™ Libraries
  • Clontech SMARTer™ PCR cDNA Synthesis Kit
  • Library Evaluation
  • Testing PCR Enzymes to Improve Representation of Long cDNAs
  • 2nd Amplification (after size selection)
  • Size Distribution of Amplified cDNA from Multiple Tissues
  • Detailed Clontech Workflow for Conversion of cDNA into SMRTbell™ Libraries
  • Options for Size Fractionation
  • No Size Selection
  • BluePippin™ Size Selection
  • Size Fractionation with SageELF™ System
  • Brain Amplifed cDNA – Size Selected
  • SageELF – 12 size bins (Amplified cDNA)
  • Amplified cDNA After Size Fractionation on SageELF System
  • SageELF SMRTbell Libraries
  • Distribution of FL Reads from SageELF Fractionated Libraries
  • Detailed Clontech Workflow for Conversion of cDNA into SMRTbell™ Libraries
  • SMRTbell Template Size Selection
  • Applications
  • Transcript Identification and Annotation
  • Identification of Alternatively Spliced Isoforms
  • Targeted Sequencing
  • Allele-Specific Transcriptomes
  • Normalization
  • Experimental Design Considerations
  • How Many SMRT Cells?
  • Resources
Down Arrow

  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences
  • Speaker: Tyson Clark, Ph.D. / Pacific Biosciences