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  • Introduction
  • FDA College Park BacBio RS (Sept. 2012 - present)
  • First Published Closed Sequence
  • WGS Provides highly detailed information
  • Pepper-coated sallami outbreak 2009-10 (S. Montevideo)
  • Reference-free case snip approach
  • CFSAN Division of Microbiology's vision for Whole Genome Sequencing (WGS)
  • Role of Pacbio RS Technology
  • Do RS data improve the quality and accuracy
  • Outbreak data sets
  • Reference-based phylogentic analysis
  • Intra-Tree Comparison
  • Inter-Tree Comparison
  • Reference-based alignments: 2 or 3 platforms
  • Function
  • Reference alignment of S. Bareilly DNA Helicase IV (PacBio highlighted)
  • S. Bareilly Plasmid
  • Hybrid assembly of the S. Bareilly CFSAN000189 plasmid
  • Comparison of RS closed chromosome sequence - Genome Bareilly CFSAN000189 against MiSeq and 454
  • CFSAN Salmonella Epigenomes
  • Salmonella enterica serovar Enteritidis (SE)
  • Cantaloupe Contamination Events
  • Methylation Pattern Comparison
  • Listeria Epigenomes
  • CFSAN DM's Journey Continues...
  • Acknowledgments
  • Q & A
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  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration
  • Speaker: Peter Evans, PhD M.P.H. / US Food & Drug Administration