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  • Introduction
  • The Pneumococcos (Streptococcos pneumoniae)
  • The Pneumocci Display Great Diversity
  • Pneumococcal Genomic Plasticity
  • Population-based supra-genome
  • Sp Core and Supra-genome sizes as a function of strain #
  • Sp Supra-genome Calculations using the Finite Supra-genome Model
  • The PMEN Strains
  • PMEN1 (The First and the Worst)
  • The PMEN1 Frequently Exchange DNA, but Primarily Among Themselves
  • Hypothesis*
  • Schematic of a Restriction-Modification System (restriction enzyme and methylase)
  • Highly Conserved Motifs for 5meC Methylases in PMEN1 Strain 23F
  • Characterization of the PMEN1 RM System RE and Methylase
  • Digestion of small DNA fragments GATC Site
  • Pyrosequencing of the Fragments GATC Sites
  • Characterization of the PMEN1 RM System
  • Methylation Site 5 methyl cytosine
  • Methylome Analysis using PacBio SMRT Sequencing
  • Comparison of PMEN1 WT and GATC Methylase KO Strains using PacBio IPD Analysis
  • Kinetic Distributions
  • Methyltransferase specificities
  • Mystery GATC Signal - Wild-type
  • We did not see the expected 5meC
  • Tet1 Treatment-Enhancement of 5meC Detection
  • Effect of Converting 5meC to 5caC on the IPD Signal
  • Enhancing the IPD Signal for 5meC by Conversion to 5caC
  • Tet1 Treatment of PMEN1 Genomic DNA
  • Sample Prep -- Bioanalyzer Traces
  • Sequencing Statistics
  • 6meA Signals do not Change with Tet1 Treatment
  • 5meC Signal is Enhanced by Tet1 Treatment
  • Motif Tables
  • GATC Tet-treated Motif Examples
  • Kinetic Signatures at GATC Sites
  • Motif Summary
  • Observations
  • Phosphorothioate
  • Dnd Locus Search in PMEN1
  • Conclusions
  • Acknowledgments and Collaborators
  • Q & A
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  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences
  • Speaker: Garth Ehrlich, PhD / Executive Director, Center for Genomic Sciences