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  • Intro
  • Acknowledgements
  • Food Security & Environmental Sustainability
  • Maize B73 Reference Assembly: Version 3
  • Data Generated
  • Assembly Strategy
  • Genome Assembly
  • Pseudomolecule Construction
  • Chr 5 Centromere
  • Diversification Rates of TE Families
  • Maize RefGenV4
  • Determination of Transcript Isoforms
  • Develop a Strategy to Optimize Transcript Discovery
  • Sequence Summary
  • Current Iso-Seq Workflow: Iso-Seq Cluster
  • Transcript Strategy
  • PacBio Iso-Seq Improves Coverage of Isoforms
  • PacBio Iso-Seq Improves Capture of Longer Transcripts & Novel Isoforms
  • Validation of Iso-Seq Transcripts by Short-Reads Sequencing
  • Structural Mis-Annotations Identified by Split Gene Models were Corrected by PacBio Iso-Seq Transcripts
  • Transcript Diversity
  • One Gene, Two Isoforms with Opposite Effects in Mouse
  • Point Mutation at the 5' Splice Site Leads to Skipping of Exon 5 and a Nonfunctional AP3 Protein
  • Functional Diversity of Isoforms From 57 Maize TFs Families
  • Example of Alternative Splicing Events
  • Alternative Splicing Events Among Different Tissues
  • Improving Genome Annotation
  • Large Intron Retention Breaks are Problematic in Automated Annotation
  • Annotation Summary Statistics Protein Coding
  • Novel Long Non-Coding RNA (LncRNA) Identification
  • Moving from a Single Reference, to Population Variation, & Functional Network Inference
  • Summary
  • Acknowledgements
Down Arrow
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory
  • Speaker: Doreen Ware, Adjunct Associate Professor / USDA Agricultural Research Service and Cold Spring Harbor Laboratory