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  • Introduction
  • Prokaryotic Functional Genomics
  • Gene Function Annotation Using TnSeq
  • Problem: Existing approaches fail to annotate gene function
  • Gene Function Annotation by TnSeq
  • Functional Encyclopedia of Bacteria and Archaea (FEBA)
  • Summary of Gene Phenotype Results from 12 Bugs
  • Identification of Regulatory DNA Methylation using SMRT Sequencing
  • Function of DNA Methylation in Prokaryotes is not Fully Known
  • Prokaryotic DNA Methylation can be Directly Detected by PacBio Sequencing
  • The Epigenomic Landscape of Bacteria
  • Overview of 232 Prokaryotic Methylomes
  • Many Active MTases are ‘orphans’
  • ‘Orphan’ MTases Have Methylation Patterns Consistent with Regulatory Roles
  • Methylation may Play a Widespread Role in Gene Regulation in Bacteria
  • Summary
  • DOE Joint Genome Institute: 
Next Generation Genome Science User Facility
  • If you shorten the growth conditions, can you stratify the lethality of genes in your assay?
  • Have you looked at the gene expression for those genes that lacked methylation?
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  • Speaker: Matthew Blow, PhD / D.O.E. Joint Genome Institute
  • Speaker: Matthew Blow, PhD / D.O.E. Joint Genome Institute
  • Speaker: Matthew Blow, PhD / D.O.E. Joint Genome Institute
  • Speaker: Matthew Blow, PhD / D.O.E. Joint Genome Institute
  • Speaker: Matthew Blow, PhD / D.O.E. Joint Genome Institute
  • Speaker: Matthew Blow, PhD / D.O.E. Joint Genome Institute
  • Speaker: Matthew Blow, PhD / D.O.E. Joint Genome Institute
  • Speaker: Matthew Blow, PhD / D.O.E. Joint Genome Institute
  • Speaker: Matthew Blow, PhD / D.O.E. Joint Genome Institute
  • Speaker: Matthew Blow, PhD / D.O.E. Joint Genome Institute
  • Speaker: Matthew Blow, PhD / D.O.E. Joint Genome Institute
  • Speaker: Matthew Blow, PhD / D.O.E. Joint Genome Institute
  • Speaker: Matthew Blow, PhD / D.O.E. Joint Genome Institute
  • Speaker: Matthew Blow, PhD / D.O.E. Joint Genome Institute
  • Speaker: Matthew Blow, PhD / D.O.E. Joint Genome Institute
  • Speaker: Matthew Blow, PhD / D.O.E. Joint Genome Institute
  • Speaker: Matthew Blow, PhD / D.O.E. Joint Genome Institute
  • Speaker: Matthew Blow, PhD / D.O.E. Joint Genome Institute
  • Speaker: Matthew Blow, PhD / D.O.E. Joint Genome Institute