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  • Introduction
  • Some Important Functions of MHC Molecules, HLA in Human
  • Molecular Mismatches are Clinically Significant
  • Over 6.5 million Samples typed by SBT and New Alleles Found at Histogenetics
  • Current Sequencing Technologies
  • Exon Antigen Recognition Sites
  • Exon-Targeted Amplicon-Based Sequencing on MiSeq and Cappillary Sanger Sequencing Platforms
  • Sanger: HLA-B Type of an individual
  • Illumina HLA-B Type of an individual
  • PacBio HLA-B Type of an individual
  • High Throughput Whole Gene Class I and Long Range Class II Sequencing on PacBio Platform
  • Phasing and Long Reads on PacBio SMRT Sequencing Platform
  • Whole Gene typing for Class I on PacBio SMRT® Sequencing Platform
  • High Throughput High Volume Typing on PacBio Platform: 7 RSII and one Sequel
  • High Throughput High Volume Typing on PacBio Platform
  • Number of Alleles-Intronic variations Observed in 150,000 Samples Typed for Whole Gene (Class I)
  • 179 Samples had Null and Alternatively Expressed Class I Alleles in 60,000 Samples Analyzed
  • 149 Samples Had Null and Alternatively Expressed Class I Alleles Reported within common “G” groups
  • Among 60,000 Analyzed 17 Samples Had Null and Alternatively Expressed 11 Novel Variations
  • HLA-A OVERVIEW of 1172 Variants
  • Sequence diversity among 1,172 unique HLA-A alleles from 197,984 sequences.
  • HLA-A (dS (Exons)-d (introns)
  • HLA-A dNdS
  • HLA-B OVERVIEW of 1226 Variants
  • Table 2. Sequence diversity among 1,226 unique HLA-B alleles from 205,340 sequences.
  • HLA-C OVERVIEW of 1184 Variants
  • Long Range Gene typing for Class II on PacBio SMRT® Sequencing Platform
  • Resolving Phase (cis-trans) Ambiguities
  • 11 Examples of DQB1 (4 Patterns) and DPB1 (20 Patterns) Exon 2 and 3 phase ambiguity with Exon targeted Amplicon-based Illumina MiSeq platform that is resolved on PacBio Platform
  • High-Throughput   HLA Class I Whole Gene and HLA Class II Long Range Typing on Sequel Platform
  • HLA Class I whole gene amplicon sequencing on RSII and Sequel
  • HLA Class II Exon 2-3 amplicon sequencing on RSII and Sequel
  • High-Throughput  HLA Class I Whole Gene and HLA Class II Long Range Typing on Sequel Platform X4
  • Conclusions
  • Acknowledgments
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  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics
  • Speaker: Nezih Cereb, CEO & Co-founder / Histogenetics