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  • Iso-Seq™ Analysis & Beyond: Advanced Bioinformatics for Transcriptome Sequencing Using Long Reads
  • Goal of This Webinar
  • Webinar Outline
  • Defining Bioinformatics
  • Iso-Seq Bioinformatics is not One-Size-Fits-All
  • For This Webinar Demonstration…
  • The Bioinformatics Role
  • Library & Sequencing Evaluation
  • Iso-Seq Library Workflow
  • Sequencing Evaluation
  • Iso-Seq classify outputs Full-Length Reads
  • Artificial Concatemers
  • PCR chimeras
  • Demo: Library & Sequencing Evaluation
  • Library & Sequencing Evaluation: Checkpoint Summary
  • Library & Sequencing Evaluation: Sample Table
  • Running Iso-Seq Bioinformatics
  • What is the Iso-Seq Bioinformatics Pipeline?
  • Iso-Seq classify identifies Full-Length Reads
  • Iso-Seq cluster outputs High-Quality Polished Sequences
  • classify vs cluster
  • Running Iso-Seq Bioinformatics: Demo Outline
  • pbtranscript-TOFU: developer’s version
  • TOFU: Developer’s version of Iso-Seq Analysis
  • Post Analysis
  • Post Analysis is Project-Dependent
  • Iso-Seq Output Can Be Redundant
  • Post-Analysis Demo: Collapsing Redundant Transcripts
  • Post-Analysis Demo: Collapsing Redundant Transcripts
  • Post-Analysis Demo: Comparing Against Annotations
  • Fusion Gene Discovery
  • Fusion Gene Discovery: Challenges
  • Fusion Gene Discovery: Solutions
  • Fusion Gene Discovery: fusion_finder.py
  • Post-Analysis Demo: Fusion Gene Discovery
  • ncRNA Discovery
  • Post-Analysis Demo: ncRNA Discovery
  • Iso-Seq Resources
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  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
  • Speaker: Elizabeth Tseng, Ph.D. / Pacific Biosciences
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