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  • Introduction
  • Overview
  • Single Molecule, Real-Time (SMRT) DNA Sequencing
  • Key Sequencing Characteristics: Contiguity
  • Longest Read Lengths
  • P5-C3 Sequencing Chemistry
  • Key Sequencing Characteristics: Accuracy
  • Highest Consensus Accuracy
  • Key Sequencing Characteristics: Uniformity
  • Least Sequence Context Bias
  • Lack of Sequencing Bias
  • Key Sequencing Characteristics: Originality
  • Epigenome Information
  • Key Sequencing Characteristics: Summary
  • Goals for a Genome Project
  • Progress of PacBio-Only De Novo Assembly
  • Hierarchical Genome Assembly Process (HGAP)
  • Finished Microbial Genome Papers using HGAP
  • Bacteria and Archaea (~1 to 10 MB Genome)
  • Comparison of RS closed chromosome sequence-Genome Bareilly CFSAN000189 against MiSeq and 454
  • Ongoing, Large-Scale Microbial Sequencing Projects
  • Most Cost-Effective Solution
  • PacBio-Only De Novo Sequencing of Yeast (12 MB)
  • PacBio-Only Arabidopisis De Novo Assembly (120 MB)
  • 200,000+ SNPs were Missed in Short-Read Assemblies
  • PacBio Drosophila De Novo Assembly (~170 MB)
  • Drosophila Y Chromosome
  • Rice Genome Assemblies
  • Illumina Library Preparation (8 libraries)
  • PacBio-Only Assembly of the Spinach Genome (~1 Gb)
  • Where We Were Before
  • Progress of PacBio-Only De Novo Assembly
  • Long-Read Shotgun Human Genome Data Release
  • Human Genome De Novo Assemblies Comparison
  • Comparison of Human CHM1 Assemblies
  • Progress of PacBio-Only De Novo Assembly
  • PacBio Long Reads Are Already Improving These Genomes
  • Structural Variation Detection
  • Structural Variation Detection by 2nd Gen Technologies
  • Complex Regions in the Human Genome
  • Resolving Complex Repetitive Regions
  • Short Tandem Repeats
  • Sequencing the Fragile X Gene
  • Resolving 'Difficult-to-Sequence' Regions
  • AGG "interruptions" reduce chances of pre-to-full mutation transmission
  • Traditional Workflow for HLA Typing
  • PacBio's Solution
  • Current State of Transcript Assembly
  • Transcript Diversity
  • One Gene, Two Isoforms with Opposite Effects
  • Difficulties for Resolving Transcripts with Short Reads
  • PacBio Iso-Seq for High-quality, Full-length Transcripts
  • ABRF NGS RNA-Seq Study: PacBio Isoform Sequencing Provides the Best 5' to 3' Coverage
  • Sequencing Full-Length Isoforms in the Human Transcriptome
  • A Novel Multi-Exonic RNA in Stem Cells
  • Annotation of Isoform Structure Improves Quantification of Isoform Expression
  • Missing Isoform Knowledge Can Bias RNA-Seq Quantification
  • Improved Quantification of Isoform Expression
  • Long-Read Sequencing of Chicken Transcripts and Identification of New Transcript Isoforms
  • Characterization of Complex Human Splice Variants
  • Splice Landscape of Neurexin 1∝
  • Other Targeted Iso-Seq Publications
  • MCF-7 Data Release
  • High-Quality Genomes & Transcriptomes
  • Other Applications
  • Quantifying Genome Editing Outcomes
  • Base Modification Detection using PacBio Sequencing
  • Number of MTases Characterized
  • Example - Helicobacter pylori Methylomes
  • Differential Methylation of Homologous Sequences
  • Add a New Dimension to Your Research
  • Technology Roadmap
  • Sample Preparation
  • Sequencing
  • Data Analysis
  • Acknowledgments
  • Q & A: Error Rate in 54 X Data Set
  • Q & A: How Complex is the Library Preparation
  • Q & A: Minimal Input For PacBio Transcriptome
  • Q & A: Read Depth and Transcriptome Size
  • Q & A: 54 X Long Read Data Alignment
  • Q & A: RNA Seq for Quantification
  • Q & A: Is Single Cell Transcriptome Sequencing Possible
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  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio
  • Speaker: Jonas Korlach, PhD / PacBio