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  • Introduction
  • Overview
  • Genetic Diversity
  • Previous approaches
  • The Solution
  • Actual longitudinal HIV env data
  • Sequencing full-length envelope: The amplicon
  • Sequencing full-length envelope: The library prep
  • HIV and the broadly neutralizing antibody puzzle
  • HIV-1 Envelope
  • Antibody/Envelope co-evolution
  • The Protocol C Cohort
  • Strategy for individual donors
  • Donor PC64
  • Phylogeny
  • Donor PC39
  • Phylogeny
  • How do the two founder variants differ?
  • Multiplexed long-read NGS for studying entire cohorts
  • Multiplexing
  • Reliability at 6-fold multiplexed depth
  • How is this going to look on the Sequel?
  • FLEA: the Full-Length Envelope Analysis pipeline
  • Other directions
  • Hot off the RS-II: Full-length HCV
  • Sequencing pol for HIV drug resistance
  • Acknowledgements
  • Q&A
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  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego
  • Speaker: Ben Murrell, Postdoctoral Fellow / Center for AIDS Research, University of California, San Diego