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  • Intro
  • Short Read Versus Long-Read Technology
  • Long Read Technology: Substantially Different Approach
  • Continuous Addition of Nucleotides
  • Principal Error Modes
  • Alignment of a PacBio Read to Reference
  • Increasing Depth of Random Error-Prone Reads Overcomes Difficulties that Increased Depth of Non-Random, Low Error Reads Cannot
  • Using Long Reads for Improved Genome Assemblies
  • Mammalian Genome Assembly Process
  • Comparison of Short-Read-Only and Long-Read-Only De Novo Assemblies : Initial Contigs
  • Scaffolding
  • Hybrid Assembly Strategies
  • Ongoing Livestock Long-Read Reference Assembly Projects
  • Ongoing Genome Assemblies
  • Goat Genome
  • Hereford Cow
  • Cattle Genome
  • Pipeline
  • Bovine Assembly Statistics
  • Ongoing Genome Assemblies: Rambouillet Sheep
  • Sheep Genome
  • Duroc / Landrace / Yorkshire
  • Pig Genome
  • Impact: e.g. RefSeq RNA Positions
  • Assembly Comparison of Porcine Dystrophin Gene
  • Assembly Comparison using Dystrophin cDNA
  • Assembly Comparison : IGF2 Region
  • Assembly Comparison: Unassigned QTL for PCV2B Viral Load
  • 19 Mb Scaffold from Chr. 12 has the SNP
  • Whole-Genome Reseq Study to Identify QTL for BVDV Persistent Infection
  • Candidate Gene on BTA 1 for BVDV P.I.
  • Fixing Assembly Causes the Signal to Entirely Disappear
  • All the other Signals also Fall in Regions of Poor Assembly
  • More General Impact for GWAS and WGR Studies
  • Increased Contiguity Improves Annotation
  • SMRT Sequence Provides Modification Data
  • Summary
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  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS
  • Speaker: Tim Smith, Research Chemist / USDS –ARS