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  • Introduction
  • Sequencing the Spinach Genome (980 Mbp)
  • Spinach
  • Why Spinach?
  • Peronospora farinosa f. sp. spinaciae
  • Illumina Sequencing
  • PacBio (60X)
  • Spinach Sequencing Yield per SMRTcell
  • Number of Reads vs. Read Length (bases)
  • Raw Read Length Cumulative Distribution Function (60 Gb Raw Sequence)
  • Overview of The Pre-Assembly Process
  • Read Length Distribution of Error Corrected pReads
  • Assembly Workflow Summary
  • Assembled Sequence in Fragments > x-axis
  • Preliminary Assembly Statistics Comparison
  • Large PacBio Contigs vs. Large BGI Scaffolds
  • Filling Gaps in Illumina Short Read Assembled Scaffold
  • Extra Bases in Illumina Short Read Assembled Scaffold
  • Structure Level Difference Observed
  • Accuracy of Assembly Relative to Illumina Assembly
  • Contiguous Alignment Blocks vs. Concordance (%)
  • Acknowledgments
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  • Speaker: Allen Van Deynze, Director of Research / UC Davis, Seed Biotechnology Center
  • Speaker: Allen Van Deynze, Director of Research / UC Davis, Seed Biotechnology Center
  • Speaker: Allen Van Deynze, Director of Research / UC Davis, Seed Biotechnology Center
  • Speaker: Allen Van Deynze, Director of Research / UC Davis, Seed Biotechnology Center
  • Speaker: Allen Van Deynze, Director of Research / UC Davis, Seed Biotechnology Center
  • Speaker: Allen Van Deynze, Director of Research / UC Davis, Seed Biotechnology Center
  • Speaker: Allen Van Deynze, Director of Research / UC Davis, Seed Biotechnology Center
  • Speaker: Allen Van Deynze, Director of Research / UC Davis, Seed Biotechnology Center
  • Speaker: Allen Van Deynze, Director of Research / UC Davis, Seed Biotechnology Center
  • Speaker: Allen Van Deynze, Director of Research / UC Davis, Seed Biotechnology Center
  • Speaker: Allen Van Deynze, Director of Research / UC Davis, Seed Biotechnology Center
  • Speaker: Allen Van Deynze, Director of Research / UC Davis, Seed Biotechnology Center
  • Speaker: Allen Van Deynze, Director of Research / UC Davis, Seed Biotechnology Center
  • Speaker: Allen Van Deynze, Director of Research / UC Davis, Seed Biotechnology Center
  • Speaker: Allen Van Deynze, Director of Research / UC Davis, Seed Biotechnology Center
  • Speaker: Allen Van Deynze, Director of Research / UC Davis, Seed Biotechnology Center
  • Speaker: Allen Van Deynze, Director of Research / UC Davis, Seed Biotechnology Center
  • Speaker: Allen Van Deynze, Director of Research / UC Davis, Seed Biotechnology Center
  • Speaker: Allen Van Deynze, Director of Research / UC Davis, Seed Biotechnology Center
  • Speaker: Allen Van Deynze, Director of Research / UC Davis, Seed Biotechnology Center
  • Speaker: Allen Van Deynze, Director of Research / UC Davis, Seed Biotechnology Center
  • Speaker: Allen Van Deynze, Director of Research / UC Davis, Seed Biotechnology Center